Publications and patents 論文・特許

Haga K, Takai-Todaka R, Matsumura Y, Song C, Takano T, Tojo T, Nagami A, Ishida Y, Masaki H, Tsuchiya M, Ebisudani T, Sugimoto S, Sato T, Yasuda H, Fukunaga K, Akihito Sawada A, Nemoto N, Murata K, Morimoto T, Katayama K., Nasal delivery of single-domain antibody improves symptoms of SARS-CoV-2 infection in an animal model. PLOS Pathogens, https://doi.org/10.1371/journal.ppat.1009542

Anzai H, Terai T, Wakabayashi-Nakao K, Noguchi T, Kumachi S, Tsuchiya M, Nemoto N., Interleukin-17A Peptide Aptamers with an Unexpected Binding Moiety Selected by cDNA Display under Heterogenous Conditions. ACS Med. Chem. Lett., 12, 1427-1434 (2021)

Jayathilake C, Kumachi S, Arai H, Motohashi M, Terai T, Murakami A, Nemoto N., In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR. Analytical Biochemistry, 589, 113490 (2020)

Arai H, Kumachi S, Nemoto N., cDNA Display: A Stable and Simple Genotype-Phenotype Coupling Using a Cell-Free Translation System. Methods in Mol. Biol., 2070, 43-56, 2020

Nemoto N, Kumachi S, Arai H., In vitro Selection of Single-Domain Antibody (VHH) using cDNA display, “Antibody Engineering 3rd Edition (edited by D. Nevoltis & P. Chames)”, Methods in Mol. Biol., 1827, 269-285 (2018)

Suzuki T, Nochizuki Y, Kimura S, Akazawa-Ogawa Y, Hagihara Y, Nemoto N., Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display, Biochem. Biophys. Res. Commun., 503, 3, 2054-2060 (2018)

寺井琢也, 熊地重文, 根本直人, 「cDNAディスプレイ法によるペプチドスクリーニング技術」, 技術情報協会「ペプチド医薬品のスクリーニング・安定化・製剤化技術」第１章, 第６節, 40-49 (2017)

Kobayashi S, Terai T, Yoshikawa Y, Ohkawa R, Edihara M, Hayashi M, Takiguchi K, Nemoto N., In vitro selection of random peptides against artificial lipid bilayers: a potential tool to immobilize molecules on membranes, Chem. Commun. (Camb.), 53, 3458-3461 (2017)

熊地重文, 根本直人, 「試験管内進化を加速するcDNA displayシステム」酵素工学ニュース, 76, 21-25 (2016)

根本直人, 「cDNAディスプレイによる機能性ペプチドアプタマーの創生」 日本生物工学会誌, 94, 481-484 (2016)

Kumachi S, Husimi Y, Nemoto N., An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display. ACS Omega. 1, 52-57 (2016)

Mochizuki Y, Suzuki T, Fujimoto K, Nemoto N., A Versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display. J. Biotechnol. 212, 174-180 (2015)

Tanemura Y, Mochizuki Y, Kumachi S, Nemoto N., Easy and rapid binding assay for fanctional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system, Biology (Basel), 4, 161-172 (2015)

Nemoto N, Fukushima T, Kumachi S, Suzuki M, Nishigaki K, kubo T., Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interations, Anal. Chem., 86, 8535-8540 (2014)

Mochizuki Y, Nisigaki K, Nemoto N., Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA display., Chem. Commun. (Camb.), 50, 5608-5610 (2014)

根本直人, 「遺伝子型-表現型対応付け技術」 (株)エヌ・ティー・エス 『進化分子工学-高速分子進化によるタンパク質・核酸の開発-』第２編, 第３章, 161-170 (2013)

根本直人, 望月佑樹, 上野真吾 「cDNA displayによる分子デザイン -mRNA display(in vitro virus)からcDNA displayへ-」 生物物理, 53, 250-253 (2013)

Mochizuki Y, Kohno F, Nishigaki K, Nemoto N., A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin linker. Anal. Biochem., 434, 93-95 (2013)

Ueno S, Kimura S, Ichiki T, Nemoto N., Improvement of a puromycin-linker to extend the selestion target varieties in cDNA display method. J. Biotechnol., 162, 299-302 (2012)

Ueno S, Yoshida S, Mondal A, Nishina K, Koyama M, Sakata I, Miura K, Hayashi Y, Nemoto N, Nishigaki K, Sakai T., In vitro selestion of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. Proc. Natl. Acad. Sci. USA, 109, 11121-11126 (2012)

Nemoto N, Tsutsui C, Yamaguchi J, Ueno S, Machida M, Kobayashi T, Sakai T., Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on inetrleukin-6-dependent cell proliferation. Biochem. Biophys. Res. Commun.. 421, 129-133 (2012)

Ueno S, Nemoto N., cDNA display: rapid stablilization of mRNA display. Methods in Mol. Biol., 805, 113-135 (2012)

Mochizuki Y, Nemoto N., Evolution of disulfide-rich peptide aptamers using cDNA display. Methods in Mol. Biol., 805, 237-250 (2012)

Mochizuki Y, Biyani M, Tsuji-Ueno S, Suzuki M, Nishigaki K, Husimi Y, Nemoto N., One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA. ACS Comb. Sci., 13, 478-485 (2011)

Naimuddin M, Kobayashi S, Tsutsui C, Machida M, Nemoto N, Sakai T, Kubo T., Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol. Brain., 4, 2 (2011)