High-throughput screening platform

cDNA Display Screening

Our HTS technology is based on cDNA display which is our advanced display method cDNA display is one of the best display methods for screening because it can handle huge library size (10¹³⁻¹⁴), and select hit molecules from the library. Also, it allows us to obtain functional clones (VHH/peptides) which have high affinity.

The one cycle of cDNA display screening includes synthesis of library (protein coeducated cDNA), selection of functional displayed proteins (Binding for target molecule) and amplify concentrated cDNA display molecules. Repeating these steps is important for screening hit clones which have high affinity for target molecules.

VHH HTS screening platform “The Month”: “The Month” is our HTS platform which is the combination of PharmaLogical® Library (For more information about PharmaLogical® Library, Click here) and cDNA display method. Also, the VHH which obtained in “The Month” platform is called PharmaLogical®VHH.The advantage of this platform is that it only takes approximately a month for obtaining hit PharmaLogical®VHHs (clones have affinity) and data of stability of each hit clone. Currently, we have been utilizing this platform for both our pipelines and joint research with pharmaceutical companies. From our past research (both our pipelines and joint research), we successfully obtained hit PharmaLogical®VHHs for targets which were not able to be obtained with conventional libraries and screening methods. Also, these hit PharmaLogical®VHHs are very stable and have high affinity for target molecules.

What “The Month” Provides you

Amino acid sequences of HitPharmaLogical®VHHs
Data of binding affinity for each hit PharmaLogical®VHHs
Data of thermal stability for each hit PharmaLogical®VHHs
Cross reactivity
Epitope grouping*
Affinity Maturation*
Prediction of immunogenicity*

*Additional options for “The Month”